gfp booster Search Results


anti resource source identifier chromotek gfp-booster alexafluor 488 proteintech gb2af488  (Proteintech)
96
Proteintech anti resource source identifier chromotek gfp-booster alexafluor 488 proteintech gb2af488
Anti Resource Source Identifier Chromotek Gfp Booster Alexafluor 488 Proteintech Gb2af488, supplied by Proteintech, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 96 stars, based on 1 article reviews
anti resource source identifier chromotek gfp-booster alexafluor 488 proteintech gb2af488 - by Bioz Stars, 2026-09
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gfp booster  (Proteintech)
96
Proteintech gfp booster
(A) sgNTC (Ametrine + )- and sg FIcn <t>(GFP</t> + )-transduced OT-I cells from indicated tissues of same host mice were profiled by ATAC-seq at day 7.5 p.i. Footprinting analysis in sg FIcn - versus sgNTC-transduced OT-I cells is shown. (B) Indicated sgRNA-transduced OT-I cells in siIEL at day 7.5 p.i. were profiled for transcriptome analysis. GSEA enrichment plot of Tfeb putative target genes comparing sg FIcn - versus sgNTC-transduced OT-I cells. FDR, false discovery rate; NES, normalized enrichment score. (C) Intracellular LAMP-1 expression (relative to spike; based on mean fluorescence intensity [MFI]) of indicated sgRNA-transduced OT-I cells in siIEL at days 4.5–5.5 p.i. ( n ≥ 4 per group) (from dual-color transfer system). (D) GSEA enrichment plots of Tfeb-activated signature (see ) in indicated sgRNA-transduced versus sgNTC-transduced spike siIEL OT-I cells that were profiled for transcriptome analysis at day 7.5 p.i. (from dual-color transfer system). (E) Nuclear Tfeb levels (based on MFI) in indicated sgRNA-transduced OT-I cells from siIEL or spleen at day 7.5 p.i. (from dual-color transfer system) ( n > 140 cells per group). (F) Confocal imaging analysis of nuclear Tfeb levels (based on MFI) in pre-activated OT-I cells cultured in control RPMI medium (Ctrl) or RPMI medium lacking all amino acids (aa − ) for 20 h (see ) ( n > 160 cells per group). (G) Intracellular LAMP-1 expression (relative to cells in Ctrl medium; based on MFI) in pre-activated OT-I cells cultured in RPMI medium lacking indicated amino acids for 2.5 days, with fold-change (if >1.5) relative to Ctrl medium indicated ( n = 3 technical replicates per group). (H) Intracellular LAMP-1 expression (relative to WT cells in Ctrl medium; based on MFI) in pre-activated WT or Cd4 Cre Tfeb fl/fl CD8 + T cells cultured in Ctrl and arginine-free (Arg − ) or glutamine-free (Gln − ) RPMI medium for 2.5 days ( n = 3 technical replicates per group). (I) Frequencies of CD69 + CD103 + <t>pMIG</t> <t>(mCherry</t> + ) and pMIG-Tfeb (GFP + )-transduced OT-I cells in siIEL at day 14 p.i. (from dual-color transfer system) ( n = 4 per group). (J and K) Frequencies (relative to spike) of total (J) or CD69 + CD103 + siIEL OT-I cells (K) transduced with indicated sgRNAs at days 7.5 or 11 p.i. (from dual-color transfer system) ( n ≥ 3 per group). One-way ANOVA (C, J, and K), two-tailed paired Student’s t test (E and I), two-tailed unpaired Student’s t test (F), two-way ANOVA (H). NS, not significant; * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001. Data (mean ± SEM) are compiled from or represent ≥2 experiments (C and E–K). See also .
Gfp Booster, supplied by Proteintech, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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resource source identifier antibodies alpaca gfp booster atto 488 conjugated nanobody chromotek cat  (Proteintech)
93
Proteintech resource source identifier antibodies alpaca gfp booster atto 488 conjugated nanobody chromotek cat
(A) sgNTC (Ametrine + )- and sg FIcn <t>(GFP</t> + )-transduced OT-I cells from indicated tissues of same host mice were profiled by ATAC-seq at day 7.5 p.i. Footprinting analysis in sg FIcn - versus sgNTC-transduced OT-I cells is shown. (B) Indicated sgRNA-transduced OT-I cells in siIEL at day 7.5 p.i. were profiled for transcriptome analysis. GSEA enrichment plot of Tfeb putative target genes comparing sg FIcn - versus sgNTC-transduced OT-I cells. FDR, false discovery rate; NES, normalized enrichment score. (C) Intracellular LAMP-1 expression (relative to spike; based on mean fluorescence intensity [MFI]) of indicated sgRNA-transduced OT-I cells in siIEL at days 4.5–5.5 p.i. ( n ≥ 4 per group) (from dual-color transfer system). (D) GSEA enrichment plots of Tfeb-activated signature (see ) in indicated sgRNA-transduced versus sgNTC-transduced spike siIEL OT-I cells that were profiled for transcriptome analysis at day 7.5 p.i. (from dual-color transfer system). (E) Nuclear Tfeb levels (based on MFI) in indicated sgRNA-transduced OT-I cells from siIEL or spleen at day 7.5 p.i. (from dual-color transfer system) ( n > 140 cells per group). (F) Confocal imaging analysis of nuclear Tfeb levels (based on MFI) in pre-activated OT-I cells cultured in control RPMI medium (Ctrl) or RPMI medium lacking all amino acids (aa − ) for 20 h (see ) ( n > 160 cells per group). (G) Intracellular LAMP-1 expression (relative to cells in Ctrl medium; based on MFI) in pre-activated OT-I cells cultured in RPMI medium lacking indicated amino acids for 2.5 days, with fold-change (if >1.5) relative to Ctrl medium indicated ( n = 3 technical replicates per group). (H) Intracellular LAMP-1 expression (relative to WT cells in Ctrl medium; based on MFI) in pre-activated WT or Cd4 Cre Tfeb fl/fl CD8 + T cells cultured in Ctrl and arginine-free (Arg − ) or glutamine-free (Gln − ) RPMI medium for 2.5 days ( n = 3 technical replicates per group). (I) Frequencies of CD69 + CD103 + <t>pMIG</t> <t>(mCherry</t> + ) and pMIG-Tfeb (GFP + )-transduced OT-I cells in siIEL at day 14 p.i. (from dual-color transfer system) ( n = 4 per group). (J and K) Frequencies (relative to spike) of total (J) or CD69 + CD103 + siIEL OT-I cells (K) transduced with indicated sgRNAs at days 7.5 or 11 p.i. (from dual-color transfer system) ( n ≥ 3 per group). One-way ANOVA (C, J, and K), two-tailed paired Student’s t test (E and I), two-tailed unpaired Student’s t test (F), two-way ANOVA (H). NS, not significant; * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001. Data (mean ± SEM) are compiled from or represent ≥2 experiments (C and E–K). See also .
Resource Source Identifier Antibodies Alpaca Gfp Booster Atto 488 Conjugated Nanobody Chromotek Cat, supplied by Proteintech, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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antibody gfp booster chromotek gba488 100 rrid ab 2631386  (Addgene inc)
90
Addgene inc antibody gfp booster chromotek gba488 100 rrid ab 2631386
(A) sgNTC (Ametrine + )- and sg FIcn <t>(GFP</t> + )-transduced OT-I cells from indicated tissues of same host mice were profiled by ATAC-seq at day 7.5 p.i. Footprinting analysis in sg FIcn - versus sgNTC-transduced OT-I cells is shown. (B) Indicated sgRNA-transduced OT-I cells in siIEL at day 7.5 p.i. were profiled for transcriptome analysis. GSEA enrichment plot of Tfeb putative target genes comparing sg FIcn - versus sgNTC-transduced OT-I cells. FDR, false discovery rate; NES, normalized enrichment score. (C) Intracellular LAMP-1 expression (relative to spike; based on mean fluorescence intensity [MFI]) of indicated sgRNA-transduced OT-I cells in siIEL at days 4.5–5.5 p.i. ( n ≥ 4 per group) (from dual-color transfer system). (D) GSEA enrichment plots of Tfeb-activated signature (see ) in indicated sgRNA-transduced versus sgNTC-transduced spike siIEL OT-I cells that were profiled for transcriptome analysis at day 7.5 p.i. (from dual-color transfer system). (E) Nuclear Tfeb levels (based on MFI) in indicated sgRNA-transduced OT-I cells from siIEL or spleen at day 7.5 p.i. (from dual-color transfer system) ( n > 140 cells per group). (F) Confocal imaging analysis of nuclear Tfeb levels (based on MFI) in pre-activated OT-I cells cultured in control RPMI medium (Ctrl) or RPMI medium lacking all amino acids (aa − ) for 20 h (see ) ( n > 160 cells per group). (G) Intracellular LAMP-1 expression (relative to cells in Ctrl medium; based on MFI) in pre-activated OT-I cells cultured in RPMI medium lacking indicated amino acids for 2.5 days, with fold-change (if >1.5) relative to Ctrl medium indicated ( n = 3 technical replicates per group). (H) Intracellular LAMP-1 expression (relative to WT cells in Ctrl medium; based on MFI) in pre-activated WT or Cd4 Cre Tfeb fl/fl CD8 + T cells cultured in Ctrl and arginine-free (Arg − ) or glutamine-free (Gln − ) RPMI medium for 2.5 days ( n = 3 technical replicates per group). (I) Frequencies of CD69 + CD103 + <t>pMIG</t> <t>(mCherry</t> + ) and pMIG-Tfeb (GFP + )-transduced OT-I cells in siIEL at day 14 p.i. (from dual-color transfer system) ( n = 4 per group). (J and K) Frequencies (relative to spike) of total (J) or CD69 + CD103 + siIEL OT-I cells (K) transduced with indicated sgRNAs at days 7.5 or 11 p.i. (from dual-color transfer system) ( n ≥ 3 per group). One-way ANOVA (C, J, and K), two-tailed paired Student’s t test (E and I), two-tailed unpaired Student’s t test (F), two-way ANOVA (H). NS, not significant; * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001. Data (mean ± SEM) are compiled from or represent ≥2 experiments (C and E–K). See also .
Antibody Gfp Booster Chromotek Gba488 100 Rrid Ab 2631386, supplied by Addgene inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/gfp+booster/pcDNA3%2E2%2FV5+hsa-mir-214+(Plasmid+%2326313)/10__7554_slash_elife__70588-356-38-56
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gfp booster fitc  (Proteintech)
96
Proteintech gfp booster fitc
(A) sgNTC (Ametrine + )- and sg FIcn <t>(GFP</t> + )-transduced OT-I cells from indicated tissues of same host mice were profiled by ATAC-seq at day 7.5 p.i. Footprinting analysis in sg FIcn - versus sgNTC-transduced OT-I cells is shown. (B) Indicated sgRNA-transduced OT-I cells in siIEL at day 7.5 p.i. were profiled for transcriptome analysis. GSEA enrichment plot of Tfeb putative target genes comparing sg FIcn - versus sgNTC-transduced OT-I cells. FDR, false discovery rate; NES, normalized enrichment score. (C) Intracellular LAMP-1 expression (relative to spike; based on mean fluorescence intensity [MFI]) of indicated sgRNA-transduced OT-I cells in siIEL at days 4.5–5.5 p.i. ( n ≥ 4 per group) (from dual-color transfer system). (D) GSEA enrichment plots of Tfeb-activated signature (see ) in indicated sgRNA-transduced versus sgNTC-transduced spike siIEL OT-I cells that were profiled for transcriptome analysis at day 7.5 p.i. (from dual-color transfer system). (E) Nuclear Tfeb levels (based on MFI) in indicated sgRNA-transduced OT-I cells from siIEL or spleen at day 7.5 p.i. (from dual-color transfer system) ( n > 140 cells per group). (F) Confocal imaging analysis of nuclear Tfeb levels (based on MFI) in pre-activated OT-I cells cultured in control RPMI medium (Ctrl) or RPMI medium lacking all amino acids (aa − ) for 20 h (see ) ( n > 160 cells per group). (G) Intracellular LAMP-1 expression (relative to cells in Ctrl medium; based on MFI) in pre-activated OT-I cells cultured in RPMI medium lacking indicated amino acids for 2.5 days, with fold-change (if >1.5) relative to Ctrl medium indicated ( n = 3 technical replicates per group). (H) Intracellular LAMP-1 expression (relative to WT cells in Ctrl medium; based on MFI) in pre-activated WT or Cd4 Cre Tfeb fl/fl CD8 + T cells cultured in Ctrl and arginine-free (Arg − ) or glutamine-free (Gln − ) RPMI medium for 2.5 days ( n = 3 technical replicates per group). (I) Frequencies of CD69 + CD103 + <t>pMIG</t> <t>(mCherry</t> + ) and pMIG-Tfeb (GFP + )-transduced OT-I cells in siIEL at day 14 p.i. (from dual-color transfer system) ( n = 4 per group). (J and K) Frequencies (relative to spike) of total (J) or CD69 + CD103 + siIEL OT-I cells (K) transduced with indicated sgRNAs at days 7.5 or 11 p.i. (from dual-color transfer system) ( n ≥ 3 per group). One-way ANOVA (C, J, and K), two-tailed paired Student’s t test (E and I), two-tailed unpaired Student’s t test (F), two-way ANOVA (H). NS, not significant; * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001. Data (mean ± SEM) are compiled from or represent ≥2 experiments (C and E–K). See also .
Gfp Booster Fitc, supplied by Proteintech, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/gfp+booster/FITC+Antibody/bio_rxiv__2022__04__11__487869-155-10-17
Average 96 stars, based on 1 article reviews
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gfp booster kit  (Proteintech)
93
Proteintech gfp booster kit
(A) sgNTC (Ametrine + )- and sg FIcn <t>(GFP</t> + )-transduced OT-I cells from indicated tissues of same host mice were profiled by ATAC-seq at day 7.5 p.i. Footprinting analysis in sg FIcn - versus sgNTC-transduced OT-I cells is shown. (B) Indicated sgRNA-transduced OT-I cells in siIEL at day 7.5 p.i. were profiled for transcriptome analysis. GSEA enrichment plot of Tfeb putative target genes comparing sg FIcn - versus sgNTC-transduced OT-I cells. FDR, false discovery rate; NES, normalized enrichment score. (C) Intracellular LAMP-1 expression (relative to spike; based on mean fluorescence intensity [MFI]) of indicated sgRNA-transduced OT-I cells in siIEL at days 4.5–5.5 p.i. ( n ≥ 4 per group) (from dual-color transfer system). (D) GSEA enrichment plots of Tfeb-activated signature (see ) in indicated sgRNA-transduced versus sgNTC-transduced spike siIEL OT-I cells that were profiled for transcriptome analysis at day 7.5 p.i. (from dual-color transfer system). (E) Nuclear Tfeb levels (based on MFI) in indicated sgRNA-transduced OT-I cells from siIEL or spleen at day 7.5 p.i. (from dual-color transfer system) ( n > 140 cells per group). (F) Confocal imaging analysis of nuclear Tfeb levels (based on MFI) in pre-activated OT-I cells cultured in control RPMI medium (Ctrl) or RPMI medium lacking all amino acids (aa − ) for 20 h (see ) ( n > 160 cells per group). (G) Intracellular LAMP-1 expression (relative to cells in Ctrl medium; based on MFI) in pre-activated OT-I cells cultured in RPMI medium lacking indicated amino acids for 2.5 days, with fold-change (if >1.5) relative to Ctrl medium indicated ( n = 3 technical replicates per group). (H) Intracellular LAMP-1 expression (relative to WT cells in Ctrl medium; based on MFI) in pre-activated WT or Cd4 Cre Tfeb fl/fl CD8 + T cells cultured in Ctrl and arginine-free (Arg − ) or glutamine-free (Gln − ) RPMI medium for 2.5 days ( n = 3 technical replicates per group). (I) Frequencies of CD69 + CD103 + <t>pMIG</t> <t>(mCherry</t> + ) and pMIG-Tfeb (GFP + )-transduced OT-I cells in siIEL at day 14 p.i. (from dual-color transfer system) ( n = 4 per group). (J and K) Frequencies (relative to spike) of total (J) or CD69 + CD103 + siIEL OT-I cells (K) transduced with indicated sgRNAs at days 7.5 or 11 p.i. (from dual-color transfer system) ( n ≥ 3 per group). One-way ANOVA (C, J, and K), two-tailed paired Student’s t test (E and I), two-tailed unpaired Student’s t test (F), two-way ANOVA (H). NS, not significant; * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001. Data (mean ± SEM) are compiled from or represent ≥2 experiments (C and E–K). See also .
Gfp Booster Kit, supplied by Proteintech, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 93 stars, based on 1 article reviews
gfp booster kit - by Bioz Stars, 2026-09
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gfp booster alexa fluor 488  (Proteintech)
96
Proteintech gfp booster alexa fluor 488
(A) sgNTC (Ametrine + )- and sg FIcn <t>(GFP</t> + )-transduced OT-I cells from indicated tissues of same host mice were profiled by ATAC-seq at day 7.5 p.i. Footprinting analysis in sg FIcn - versus sgNTC-transduced OT-I cells is shown. (B) Indicated sgRNA-transduced OT-I cells in siIEL at day 7.5 p.i. were profiled for transcriptome analysis. GSEA enrichment plot of Tfeb putative target genes comparing sg FIcn - versus sgNTC-transduced OT-I cells. FDR, false discovery rate; NES, normalized enrichment score. (C) Intracellular LAMP-1 expression (relative to spike; based on mean fluorescence intensity [MFI]) of indicated sgRNA-transduced OT-I cells in siIEL at days 4.5–5.5 p.i. ( n ≥ 4 per group) (from dual-color transfer system). (D) GSEA enrichment plots of Tfeb-activated signature (see ) in indicated sgRNA-transduced versus sgNTC-transduced spike siIEL OT-I cells that were profiled for transcriptome analysis at day 7.5 p.i. (from dual-color transfer system). (E) Nuclear Tfeb levels (based on MFI) in indicated sgRNA-transduced OT-I cells from siIEL or spleen at day 7.5 p.i. (from dual-color transfer system) ( n > 140 cells per group). (F) Confocal imaging analysis of nuclear Tfeb levels (based on MFI) in pre-activated OT-I cells cultured in control RPMI medium (Ctrl) or RPMI medium lacking all amino acids (aa − ) for 20 h (see ) ( n > 160 cells per group). (G) Intracellular LAMP-1 expression (relative to cells in Ctrl medium; based on MFI) in pre-activated OT-I cells cultured in RPMI medium lacking indicated amino acids for 2.5 days, with fold-change (if >1.5) relative to Ctrl medium indicated ( n = 3 technical replicates per group). (H) Intracellular LAMP-1 expression (relative to WT cells in Ctrl medium; based on MFI) in pre-activated WT or Cd4 Cre Tfeb fl/fl CD8 + T cells cultured in Ctrl and arginine-free (Arg − ) or glutamine-free (Gln − ) RPMI medium for 2.5 days ( n = 3 technical replicates per group). (I) Frequencies of CD69 + CD103 + <t>pMIG</t> <t>(mCherry</t> + ) and pMIG-Tfeb (GFP + )-transduced OT-I cells in siIEL at day 14 p.i. (from dual-color transfer system) ( n = 4 per group). (J and K) Frequencies (relative to spike) of total (J) or CD69 + CD103 + siIEL OT-I cells (K) transduced with indicated sgRNAs at days 7.5 or 11 p.i. (from dual-color transfer system) ( n ≥ 3 per group). One-way ANOVA (C, J, and K), two-tailed paired Student’s t test (E and I), two-tailed unpaired Student’s t test (F), two-way ANOVA (H). NS, not significant; * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001. Data (mean ± SEM) are compiled from or represent ≥2 experiments (C and E–K). See also .
Gfp Booster Alexa Fluor 488, supplied by Proteintech, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/gfp+booster/PTPN5+Antibody/bio_rxiv__2024__03__01__583047-287-16-20
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gfp booster  (Rockland Immunochemicals)
93
Rockland Immunochemicals gfp booster
(A) sgNTC (Ametrine + )- and sg FIcn <t>(GFP</t> + )-transduced OT-I cells from indicated tissues of same host mice were profiled by ATAC-seq at day 7.5 p.i. Footprinting analysis in sg FIcn - versus sgNTC-transduced OT-I cells is shown. (B) Indicated sgRNA-transduced OT-I cells in siIEL at day 7.5 p.i. were profiled for transcriptome analysis. GSEA enrichment plot of Tfeb putative target genes comparing sg FIcn - versus sgNTC-transduced OT-I cells. FDR, false discovery rate; NES, normalized enrichment score. (C) Intracellular LAMP-1 expression (relative to spike; based on mean fluorescence intensity [MFI]) of indicated sgRNA-transduced OT-I cells in siIEL at days 4.5–5.5 p.i. ( n ≥ 4 per group) (from dual-color transfer system). (D) GSEA enrichment plots of Tfeb-activated signature (see ) in indicated sgRNA-transduced versus sgNTC-transduced spike siIEL OT-I cells that were profiled for transcriptome analysis at day 7.5 p.i. (from dual-color transfer system). (E) Nuclear Tfeb levels (based on MFI) in indicated sgRNA-transduced OT-I cells from siIEL or spleen at day 7.5 p.i. (from dual-color transfer system) ( n > 140 cells per group). (F) Confocal imaging analysis of nuclear Tfeb levels (based on MFI) in pre-activated OT-I cells cultured in control RPMI medium (Ctrl) or RPMI medium lacking all amino acids (aa − ) for 20 h (see ) ( n > 160 cells per group). (G) Intracellular LAMP-1 expression (relative to cells in Ctrl medium; based on MFI) in pre-activated OT-I cells cultured in RPMI medium lacking indicated amino acids for 2.5 days, with fold-change (if >1.5) relative to Ctrl medium indicated ( n = 3 technical replicates per group). (H) Intracellular LAMP-1 expression (relative to WT cells in Ctrl medium; based on MFI) in pre-activated WT or Cd4 Cre Tfeb fl/fl CD8 + T cells cultured in Ctrl and arginine-free (Arg − ) or glutamine-free (Gln − ) RPMI medium for 2.5 days ( n = 3 technical replicates per group). (I) Frequencies of CD69 + CD103 + <t>pMIG</t> <t>(mCherry</t> + ) and pMIG-Tfeb (GFP + )-transduced OT-I cells in siIEL at day 14 p.i. (from dual-color transfer system) ( n = 4 per group). (J and K) Frequencies (relative to spike) of total (J) or CD69 + CD103 + siIEL OT-I cells (K) transduced with indicated sgRNAs at days 7.5 or 11 p.i. (from dual-color transfer system) ( n ≥ 3 per group). One-way ANOVA (C, J, and K), two-tailed paired Student’s t test (E and I), two-tailed unpaired Student’s t test (F), two-way ANOVA (H). NS, not significant; * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001. Data (mean ± SEM) are compiled from or represent ≥2 experiments (C and E–K). See also .
Gfp Booster, supplied by Rockland Immunochemicals, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


(A) sgNTC (Ametrine + )- and sg FIcn (GFP + )-transduced OT-I cells from indicated tissues of same host mice were profiled by ATAC-seq at day 7.5 p.i. Footprinting analysis in sg FIcn - versus sgNTC-transduced OT-I cells is shown. (B) Indicated sgRNA-transduced OT-I cells in siIEL at day 7.5 p.i. were profiled for transcriptome analysis. GSEA enrichment plot of Tfeb putative target genes comparing sg FIcn - versus sgNTC-transduced OT-I cells. FDR, false discovery rate; NES, normalized enrichment score. (C) Intracellular LAMP-1 expression (relative to spike; based on mean fluorescence intensity [MFI]) of indicated sgRNA-transduced OT-I cells in siIEL at days 4.5–5.5 p.i. ( n ≥ 4 per group) (from dual-color transfer system). (D) GSEA enrichment plots of Tfeb-activated signature (see ) in indicated sgRNA-transduced versus sgNTC-transduced spike siIEL OT-I cells that were profiled for transcriptome analysis at day 7.5 p.i. (from dual-color transfer system). (E) Nuclear Tfeb levels (based on MFI) in indicated sgRNA-transduced OT-I cells from siIEL or spleen at day 7.5 p.i. (from dual-color transfer system) ( n > 140 cells per group). (F) Confocal imaging analysis of nuclear Tfeb levels (based on MFI) in pre-activated OT-I cells cultured in control RPMI medium (Ctrl) or RPMI medium lacking all amino acids (aa − ) for 20 h (see ) ( n > 160 cells per group). (G) Intracellular LAMP-1 expression (relative to cells in Ctrl medium; based on MFI) in pre-activated OT-I cells cultured in RPMI medium lacking indicated amino acids for 2.5 days, with fold-change (if >1.5) relative to Ctrl medium indicated ( n = 3 technical replicates per group). (H) Intracellular LAMP-1 expression (relative to WT cells in Ctrl medium; based on MFI) in pre-activated WT or Cd4 Cre Tfeb fl/fl CD8 + T cells cultured in Ctrl and arginine-free (Arg − ) or glutamine-free (Gln − ) RPMI medium for 2.5 days ( n = 3 technical replicates per group). (I) Frequencies of CD69 + CD103 + pMIG (mCherry + ) and pMIG-Tfeb (GFP + )-transduced OT-I cells in siIEL at day 14 p.i. (from dual-color transfer system) ( n = 4 per group). (J and K) Frequencies (relative to spike) of total (J) or CD69 + CD103 + siIEL OT-I cells (K) transduced with indicated sgRNAs at days 7.5 or 11 p.i. (from dual-color transfer system) ( n ≥ 3 per group). One-way ANOVA (C, J, and K), two-tailed paired Student’s t test (E and I), two-tailed unpaired Student’s t test (F), two-way ANOVA (H). NS, not significant; * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001. Data (mean ± SEM) are compiled from or represent ≥2 experiments (C and E–K). See also .

Journal: Immunity

Article Title: CRISPR screens unveil nutrient-dependent lysosomal and mitochondrial nodes impacting intestinal tissue-resident memory CD8 + T cell formation

doi: 10.1016/j.immuni.2024.09.013

Figure Lengend Snippet: (A) sgNTC (Ametrine + )- and sg FIcn (GFP + )-transduced OT-I cells from indicated tissues of same host mice were profiled by ATAC-seq at day 7.5 p.i. Footprinting analysis in sg FIcn - versus sgNTC-transduced OT-I cells is shown. (B) Indicated sgRNA-transduced OT-I cells in siIEL at day 7.5 p.i. were profiled for transcriptome analysis. GSEA enrichment plot of Tfeb putative target genes comparing sg FIcn - versus sgNTC-transduced OT-I cells. FDR, false discovery rate; NES, normalized enrichment score. (C) Intracellular LAMP-1 expression (relative to spike; based on mean fluorescence intensity [MFI]) of indicated sgRNA-transduced OT-I cells in siIEL at days 4.5–5.5 p.i. ( n ≥ 4 per group) (from dual-color transfer system). (D) GSEA enrichment plots of Tfeb-activated signature (see ) in indicated sgRNA-transduced versus sgNTC-transduced spike siIEL OT-I cells that were profiled for transcriptome analysis at day 7.5 p.i. (from dual-color transfer system). (E) Nuclear Tfeb levels (based on MFI) in indicated sgRNA-transduced OT-I cells from siIEL or spleen at day 7.5 p.i. (from dual-color transfer system) ( n > 140 cells per group). (F) Confocal imaging analysis of nuclear Tfeb levels (based on MFI) in pre-activated OT-I cells cultured in control RPMI medium (Ctrl) or RPMI medium lacking all amino acids (aa − ) for 20 h (see ) ( n > 160 cells per group). (G) Intracellular LAMP-1 expression (relative to cells in Ctrl medium; based on MFI) in pre-activated OT-I cells cultured in RPMI medium lacking indicated amino acids for 2.5 days, with fold-change (if >1.5) relative to Ctrl medium indicated ( n = 3 technical replicates per group). (H) Intracellular LAMP-1 expression (relative to WT cells in Ctrl medium; based on MFI) in pre-activated WT or Cd4 Cre Tfeb fl/fl CD8 + T cells cultured in Ctrl and arginine-free (Arg − ) or glutamine-free (Gln − ) RPMI medium for 2.5 days ( n = 3 technical replicates per group). (I) Frequencies of CD69 + CD103 + pMIG (mCherry + ) and pMIG-Tfeb (GFP + )-transduced OT-I cells in siIEL at day 14 p.i. (from dual-color transfer system) ( n = 4 per group). (J and K) Frequencies (relative to spike) of total (J) or CD69 + CD103 + siIEL OT-I cells (K) transduced with indicated sgRNAs at days 7.5 or 11 p.i. (from dual-color transfer system) ( n ≥ 3 per group). One-way ANOVA (C, J, and K), two-tailed paired Student’s t test (E and I), two-tailed unpaired Student’s t test (F), two-way ANOVA (H). NS, not significant; * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001. Data (mean ± SEM) are compiled from or represent ≥2 experiments (C and E–K). See also .

Article Snippet: 10 μm thick cryosections were blocked in PBS containing 2% bovine serum albumin, 5% normal donkey serum and 0.05% Tween-20 followed by incubation with the following reagents: GFP booster (catalog #gba488, Chromotek/ ProteinTech), anti-mCherry (catalog #orb11618, Biorbyt), and biotin-labeled anti-Epcam (clone G8.8, Biolegend).

Techniques: Footprinting, Expressing, Fluorescence, Imaging, Cell Culture, Control, Transduction, Two Tailed Test

KEY RESOURCES TABLE

Journal: Immunity

Article Title: CRISPR screens unveil nutrient-dependent lysosomal and mitochondrial nodes impacting intestinal tissue-resident memory CD8 + T cell formation

doi: 10.1016/j.immuni.2024.09.013

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: 10 μm thick cryosections were blocked in PBS containing 2% bovine serum albumin, 5% normal donkey serum and 0.05% Tween-20 followed by incubation with the following reagents: GFP booster (catalog #gba488, Chromotek/ ProteinTech), anti-mCherry (catalog #orb11618, Biorbyt), and biotin-labeled anti-Epcam (clone G8.8, Biolegend).

Techniques: Purification, Virus, Expressing, Mutagenesis, Recombinant, Electron Microscopy, Control, Modification, Plasmid Preparation, Cell Isolation, Transfection, Sample Prep, Reverse Transcription, SYBR Green Assay, Microarray, RNA Sequencing, Knock-In, Sequencing, Software, Flow Cytometry, Microscopy, Real-time Polymerase Chain Reaction